Phenotype remodeling in neutrophilic granulocyte subsets CD64^-CD32⁺CD16⁺CD11B⁺NG, CD64⁺CD32⁺CD16⁺CD11B⁺NG in <i>de novo</i> experimental model of viral-bacterial infection <i>in vitro</i>

A search for new targeted therapeutic strategies based on examining immunopathogenetic mechanisms emerging co-infections is relevant and may further contribute not only to optimizing choice of immunotropic drugs, but also achieving positive clinical immunological remission abnormal infectious processes. Previously, our studies found that recurrent viral-bacterial respiratory infections are associated with dysfunction neutrophilic granulocytes (NG) varying degree intensity in altered effector properties. NG dysfunctions often diverse phenotypic profiles characterized by density expression level functionally significant trigger receptors. The aim the study was pinpoint phenotype transformation CD64-CD32+CD16+CD11b+, CD64+CD32+CD16+CD11b+ experimental model infection vitro. We examined 52 peripheral blood samples collected from 13 healthy adult volunteers. Viral, bacterial virus-bacterial modelled vitro incubating blood-derived cell formyl-methionyl-leucyl-phenylalanine (fMLP), double-stranded RNA (dsRNA) or combination followed assessing changes immunophenotyping CD64-CD32+CD16+CD11b+NG, CD64+CD32+CD16+CD11b+NG using MAbs CD16-ECD, CD64-FITC, CD32-PE, CD11b-PC5 conjugates (Beckman Coulter International SA, France). It demonstrated NGs volunteers were dominated CD64-CD32+CD16+CD11b+NG as well minor subset СD64+CD32+CD16+CD11b+ membrane molecules. Percentage СD64+CD16+CD32+CD11b+ significantly increased after exposure dsRNA, fMLP dsRNA+fMLP compared untreated samples. Comparative analysis revealed various agents differed affecting surface receptor molecules CD16, CD32 unidirectional effects, magnitude altering CD11b marker both major subsets. Preincubation dsRNA adding allowed find they co-stimulated receptors generated an co-infection observed types CD64-CD32+CD16+CD11b+ This can be used evaluate other phenotypes, functional activity, features NET formation impact NG.